What It Is
The GLOW Blend is a comprehensive healing peptide combination that brings together three powerful compounds: TB-500 (10 mg), BPC-157 (10 mg), and GHK-Cu (50 mg). This 70 mg total blend represents an advanced approach to tissue repair and regeneration by combining the Wolverine Stack (BPC-157 + TB-500) with the anti-aging and collagen-supporting properties of GHK-Cu (copper peptide).
Each component serves a distinct purpose:
TB-500 (Thymosin Beta-4): A synthetic version of a naturally occurring protein that promotes cell migration, angiogenesis, and systemic tissue repair through actin regulation.
BPC-157 (Body Protection Compound): A pentadecapeptide derived from gastric juice that promotes localized healing, growth factor signaling, and tissue protection.
GHK-Cu (Copper Peptide): A naturally occurring tripeptide that supports collagen synthesis, gene expression modulation, and skin regeneration through copper-dependent pathways.
The name "GLOW" reflects the combination's benefits for both internal healing and external appearance. While the Wolverine Stack focuses on injury recovery and tissue repair, the addition of GHK-Cu brings anti-aging, skin rejuvenation, and collagen support into the equation.
This product contains double the peptide content of the standard Wolverine Stack (10 mg each vs 5 mg each of BPC-157 and TB-500) plus a substantial 50 mg dose of GHK-Cu.
None of these peptides are FDA approved for human use. BPC-157 and TB-500 are banned by WADA under the S0 Unapproved Substances category.
How It Works
The GLOW Blend operates through multiple complementary mechanisms, with each peptide targeting different aspects of tissue repair and regeneration.
TB-500 Mechanisms:
TB-500 works systemically through actin regulation:
• Binds to and sequesters actin monomers, maintaining a reserve for rapid cell mobilization
• Promotes cell migration over long distances due to low molecular weight
• Stimulates angiogenesis (new blood vessel formation)
• Releases anti-inflammatory peptide fragment Ac-SDKP during metabolism
• Works throughout the body regardless of injection site
BPC-157 Mechanisms:
BPC-157 works as an initiator and protector:
• Upregulates VEGF to promote blood vessel formation
• Modulates nitric oxide synthesis for improved blood flow
• Activates FAK-paxillin pathways for cell migration and attachment
• Increases growth hormone receptor expression in damaged tissues
• Provides gastric protection and supports gut healing
GHK-Cu Mechanisms:
GHK-Cu works through gene expression modulation and copper delivery:
• Delivers bioavailable copper to tissues for enzyme activation
• Serves as a cofactor for lysyl oxidase (critical for collagen cross-linking)
• Stimulates fibroblast production of collagen, elastin, and glycosaminoglycans
• Modulates expression of over 4,000 genes toward healthier patterns
• Regulates metalloproteinases for balanced collagen turnover
• Provides antioxidant and anti-inflammatory effects
Synergy in the GLOW Blend:
The three peptides create a comprehensive healing environment:
1. BPC-157 initiates the healing response by calling in growth factors, protecting tissues, and establishing blood supply to damaged areas
2. TB-500 mobilizes repair cells, directs them to injury sites, and manages cellular reconstruction through actin regulation
3. GHK-Cu provides the building blocks and enzymatic support for high-quality tissue rebuilding, while modulating gene expression toward regeneration rather than scarring
The combination addresses:
• Acute tissue repair (BPC-157 + TB-500)
• Systemic healing distribution (TB-500)
• Collagen quality and skin appearance (GHK-Cu)
• Anti-aging and tissue remodeling (GHK-Cu)
• Inflammation management (all three)
• Angiogenesis support (all three through different pathways)
Benefits
The GLOW Blend combines the benefits of all three peptide components, creating a comprehensive approach to healing and regeneration.
Comprehensive Tissue Healing
The TB-500 and BPC-157 components provide the core healing benefits:
• Accelerated tendon and ligament repair
• Faster muscle recovery
• Enhanced wound healing
• Improved post-surgical recovery
• Joint support and cartilage protection
• Reduced scar tissue formation
Skin and Collagen Support
The GHK-Cu component adds:
• Increased collagen synthesis (up to 70% in laboratory studies)
• Improved skin thickness and density
• Enhanced skin elasticity and firmness
• Reduced appearance of fine lines and wrinkles
• Better skin hydration
• Support for hair growth and thickness
Anti-Aging Benefits
GHK-Cu provides gene expression modulation that:
• Shifts gene patterns toward more youthful states
• Supports DNA repair processes
• Provides antioxidant protection
• Reduces age-related tissue degradation
Systemic Distribution
TB-500's systemic properties mean the blend can:
• Support healing at multiple sites simultaneously
• Reach injuries regardless of injection location
• Provide whole-body regenerative support
Reduced Injection Site Reactions
When GHK-Cu and BPC-157 are combined, users often report reduced injection site irritation compared to GHK-Cu alone. The BPC-157 component appears to help moderate the histamine response that pure GHK-Cu can produce.
Gut Health Support
The BPC-157 component provides:
• Stomach lining protection
• Support for inflammatory bowel conditions
• Protection against NSAID-induced gut damage
What the Science Shows
Research on the individual components of the GLOW Blend is extensive. No studies have examined this specific three-peptide combination, but research on each peptide supports the rationale for combining them.
BPC-157 Research:
Gwyer et al. (2019) reviewed BPC-157's effects on musculoskeletal soft tissue healing, finding it shows promise for healing hypovascular tissues with few reported adverse reactions.
Chang et al. (2011) demonstrated BPC-157 promotes tendon healing through enhanced cell outgrowth, survival under stress, and migration via FAK-paxillin pathway activation.
TB-500 Research:
Malinda et al. (1999) showed thymosin beta-4 increased re-epithelialization by 42% at day 4 and up to 61% at day 7 in wound models, with improved wound contraction and collagen deposition.
Bock-Marquette et al. (2004) demonstrated thymosin beta-4 activates integrin-linked kinase and promotes cardiac cell migration, survival, and repair.
GHK-Cu Research:
Pickart and Margolina (2018) reviewed GHK-Cu's effects on gene expression, finding it modulates over 4,000 genes generally toward healthier patterns, affecting antioxidant defense, anti-inflammatory response, and DNA repair.
Maquart et al. (1993) showed GHK-Cu increased collagen synthesis 9-fold in treated wounds with accelerated healing.
Multiple facial studies have shown GHK-Cu increases skin density, thickness, firmness, and reduces fine lines over 8 to 12 week treatment periods.
Sources:
• Gwyer D, et al. Cell Tissue Res. 2019. https://pubmed.ncbi.nlm.nih.gov/30915550/
• Chang CH, et al. J Appl Physiol. 2011. https://pubmed.ncbi.nlm.nih.gov/21030672/
• Malinda KM, et al. J Invest Dermatol. 1999. https://pubmed.ncbi.nlm.nih.gov/10469335/
• Bock-Marquette I, et al. Nature. 2004. https://pubmed.ncbi.nlm.nih.gov/15565145/
• Pickart L, Margolina A. Int J Mol Sci. 2018. https://pubmed.ncbi.nlm.nih.gov/30002323/
• Maquart FX, et al. J Clin Invest. 1993. https://pubmed.ncbi.nlm.nih.gov/8227361/
Dosing Protocol
The GLOW Blend (70 mg total) contains TB-500 10 mg + BPC-157 10 mg + GHK-Cu 50 mg. Dosing is based on dividing this total appropriately across your protocol duration.
Standard Protocol
Frequency: Daily or every other day subcutaneous injection Cycle length: 4 to 8 weeks for healing focus, 8 to 16 weeks for anti-aging focus Break: 4 to 6 weeks between cycles
Dose Calculation
With 70 mg total in the vial, common approaches include:
Daily injection approach (for 8 week cycle):
• Reconstitute with 3.5 mL bacteriostatic water
• Inject 0.5 mL daily
• Each injection delivers approximately 357 mcg BPC-157 + 357 mcg TB-500 + 1.79 mg GHK-Cu
• Vial duration: approximately 7 days
Every other day approach (for extended use):
• Reconstitute with 3.5 mL bacteriostatic water
• Inject 0.5 mL every other day
• Vial duration: approximately 14 days
Twice weekly approach (maintenance):
• Reconstitute with 3.5 mL bacteriostatic water
• Inject 0.5 to 1.0 mL twice weekly
• Allows extended vial duration
By Application
Injury recovery plus appearance:
• Daily injection for 4 to 6 weeks
• Addresses both healing and skin quality
Anti-aging and maintenance:
• Every other day or 3 times weekly for 8 to 16 weeks
• Focus on collagen support and gene expression modulation
Post-surgical with skin healing:
• Daily injection starting post-surgery (with surgeon approval)
• Supports wound healing and minimizes scarring
Administration
The GLOW Blend can be injected subcutaneously anywhere due to TB-500's systemic distribution. Common sites include abdomen, thigh, or upper arm. The GHK-Cu component will produce a blue-tinted solution, which is normal.
Draw Volumes by Vial Size
70 mg Vial with 3.5 mL Bacteriostatic Water (20 mg/mL total concentration)
Each mL contains:
• BPC-157: 2.86 mg (2,860 mcg)
• TB-500: 2.86 mg (2,860 mcg)
• GHK-Cu: 14.29 mg
Volume BPC-157 TB-500 GHK-Cu Units
──────────────────────────────────────────────────────────────
0.25 mL 714 mcg 714 mcg 3.57 mg 25 units
0.35 mL 1,000 mcg 1,000 mcg 5.0 mg 35 units
0.50 mL 1,430 mcg 1,430 mcg 7.14 mg 50 units
At 0.5 mL daily, one vial lasts 7 days. At 0.35 mL daily, one vial lasts 10 days. At 0.25 mL daily, one vial lasts 14 days.
Alternative Reconstitution (2.5 mL for higher concentration)
Each mL contains:
• BPC-157: 4 mg (4,000 mcg)
• TB-500: 4 mg (4,000 mcg)
• GHK-Cu: 20 mg
This concentration allows smaller injection volumes.
Reconstitution
Materials Needed:
• GLOW Blend vial (lyophilized powder)
• Bacteriostatic water
• Sterile syringe for reconstitution
• Alcohol swabs
Instructions:
1. Wipe the vial stopper and bacteriostatic water vial with alcohol swabs
2. Draw 2.5 to 3.5 mL of bacteriostatic water (depending on desired concentration)
3. Insert needle through rubber stopper at an angle
4. Let water trickle slowly down the inside wall of the vial
5. Do not inject directly onto the powder or shake vigorously
6. Gently swirl until fully dissolved
7. The solution will have a distinctive blue color due to the GHK-Cu copper content
8. If solution is not blue or contains particles, do not use
The blue color is normal and indicates the copper is properly bound to the GHK peptide.
Side Effects
The GLOW Blend combines three peptides with favorable safety profiles. Side effects are generally mild and similar to those reported with individual components.
Common:
• Injection site reactions including redness, swelling, or itching (primarily from GHK-Cu component)
• Mild fatigue or lethargy
• Nausea (uncommon)
• Headache (uncommon)
GHK-Cu Specific:
The GHK-Cu component can cause histamine responses at injection sites (redness, itching, small bumps). However, when combined with BPC-157, these reactions are often less pronounced than with GHK-Cu alone.
Strategies to Minimize Injection Site Reactions:
• Rotate injection sites (never use the same spot twice in a row)
• Allow the solution to warm to room temperature before injecting
• Inject slowly
• Apply light pressure after injection
Theoretical Concerns:
All three peptides promote angiogenesis through different pathways. This raises theoretical concerns about use in individuals with existing cancers or tumors. No evidence has demonstrated this risk, but caution is warranted.
Contraindications and Precautions
Do Not Use If You Have:
• Active cancer or tumors (theoretical concern about angiogenesis)
• History of malignancy
• Known hypersensitivity to BPC-157, TB-500, thymosin peptides, or copper
• Wilson's disease (genetic copper accumulation disorder)
Use Caution With:
• Pregnancy or breastfeeding (no safety data)
• Severe cardiovascular conditions
• Autoimmune conditions
• Immunosuppressant medications
• Liver or kidney impairment
Drug Interactions:
Limited data available. Consult healthcare provider if taking medications.
Regulatory Status:
• BPC-157 and TB-500: FDA Category 2 bulk drug substances, WADA prohibited
• GHK-Cu: Widely used in cosmetics (Copper Tripeptide-1), injectable forms restricted
• Available as research chemical
Consult a qualified healthcare provider before use.
Comparison Table
Product Contents Best For Price
──────────────────────────────────────────────────────────────────────────────────
GLOW Blend TB-500 10mg + Comprehensive healing $99.99
BPC-157 10mg + + anti-aging
GHK-Cu 50mg + skin/collagen
Wolverine Stack BPC-157 5mg + Injury recovery $49.99
TB-500 5mg Post-surgical healing
KLOW Blend TB-500 + BPC-157 + Maximum healing $119.99
GHK-Cu + KPV + gut inflammation
+ anti-aging
GHK-Cu Alone 50mg Anti-aging $34.99
Skin/collagen focus
When to Choose GLOW vs Wolverine Stack:
Choose GLOW Blend if:
• You want injury recovery plus anti-aging and skin benefits
• You want stronger doses of BPC-157 and TB-500 (10 mg each vs 5 mg each)
• Collagen support and skin quality are priorities
• You prefer a comprehensive single-vial solution
Choose Wolverine Stack if:
• Your main goal is pure injury recovery
• You want a simpler, more affordable protocol
• Anti-aging and skin appearance are not priorities
When to Choose GLOW vs KLOW:
Choose GLOW if:
• You want healing plus anti-aging without gut-specific peptides
• You do not need KPV for gut inflammation
Choose KLOW if:
• You have significant gut inflammation or IBD concerns
• You want maximum comprehensive support
Success Tips
Expect Gradual Results
The GLOW Blend works through multiple mechanisms with different timelines:
• Injury healing improvements: 1 to 4 weeks
• Skin texture and hydration: 2 to 4 weeks
• Significant collagen/firmness improvements: 8 to 12 weeks
The Blue Color is Normal
Reconstituted GLOW Blend will have a blue tint from the GHK-Cu component. This is normal and indicates the copper is properly bound. If your solution is not blue, there may be an issue with the product.
Reduced Site Reactions
One benefit of the GLOW Blend vs standalone GHK-Cu is that BPC-157 appears to moderate injection site reactions. If you have previously experienced irritation with GHK-Cu alone, you may find the blend more tolerable.
Injection Site Selection
Because TB-500 works systemically, you can inject the GLOW Blend anywhere convenient. However, if you have a specific injury, injecting near that area may maximize BPC-157's localized effects while still receiving systemic benefits.
Combine With Good Fundamentals
Peptides enhance your body's healing capacity, but they work best alongside:
• Adequate protein intake (supports collagen synthesis)
• Vitamin C (essential cofactor for collagen production)
• Quality sleep (when tissue repair is most active)
• Appropriate rest and rehabilitation for injuries
Need Help With Training or Nutrition?
Peptides support recovery and appearance, but they work best alongside proper training and nutrition. If you would like more information, please check out our website at: turawellness.com
Injection Technique
The GLOW Blend is administered via subcutaneous injection.
Subcutaneous Injection:
1. Wash hands thoroughly with soap and water
2. Clean the vial stopper with an alcohol swab and allow to air dry
3. Draw the appropriate dose into a sterile insulin syringe (29 to 31 gauge)
4. Clean the injection site with an alcohol swab
5. Pinch a fold of skin
6. Insert needle at 45 to 90 degree angle
7. Inject slowly and steadily
8. Release skin fold and withdraw needle
9. Apply light pressure with gauze if needed
10. Dispose of syringe immediately in a sharps container
Site Selection:
Common injection sites:
• Abdomen (stay 2 inches from navel)
• Outer thigh
• Back of upper arm
• Near injury site (if targeting specific area)
Rotate injection sites to avoid tissue irritation.
Storage and Handling
Before Reconstitution:
• Store lyophilized (powder) vials in the refrigerator at 36 to 46 degrees Fahrenheit (2 to 8 degrees Celsius)
• Can be stored in freezer for longer-term storage
• Protect from light
After Reconstitution:
• Refrigerate at 36 to 46 degrees Fahrenheit
• Use within 4 weeks when reconstituted with bacteriostatic water
• Do not freeze after reconstitution
• Keep the stopper clean
• The blue color should remain stable. If color changes or particles appear, discard
Legal Status
United States:
BPC-157 and TB-500 are not FDA approved for human use and were classified as Category 2 bulk drug substances in 2023. GHK-Cu is widely used in cosmetic products but injectable forms face similar restrictions. The GLOW Blend is available as a research chemical.
WADA Status:
BPC-157 and TB-500 are prohibited under the S0 Unapproved Substances category. Athletes subject to drug testing should not use this combination.
Frequently Asked Questions
Why choose GLOW Blend over the Wolverine Stack?
The GLOW Blend adds GHK-Cu (50 mg) to the Wolverine Stack formula and doubles the BPC-157 and TB-500 doses (10 mg each vs 5 mg each). Choose GLOW if you want injury healing plus anti-aging, skin improvement, and collagen support. Choose Wolverine Stack if you only need injury recovery.
What is the blue color in the solution?
The blue color comes from the GHK-Cu (copper peptide) component and is completely normal. It indicates the copper is properly bound to the peptide. If your solution is not blue, the product may be compromised.
Will the GLOW Blend help with skin appearance?
Yes. The GHK-Cu component supports collagen synthesis, skin thickness, firmness, and elasticity. Multiple clinical studies have shown GHK-Cu improves these parameters over 8 to 12 weeks of consistent use. The BPC-157 and TB-500 components also support wound healing and tissue quality.
Can I use GLOW Blend for gut issues?
While the BPC-157 component provides some gut support when injected, standalone oral BPC-157 is more targeted for gut-specific issues. If gut inflammation is your primary concern, consider the KLOW Blend (which adds KPV for gut inflammation) or oral BPC-157.
How long should I run the GLOW Blend?
For injury healing: 4 to 8 weeks For anti-aging and collagen support: 8 to 16 weeks Take 4 to 6 weeks off between cycles.
Product Source
Research Grade GLOW Blend available at turawellness.com
Disclaimer
This guide provides educational information about the GLOW Blend (TB-500 + BPC-157 + GHK-Cu) based on published scientific literature and practical community experience. This is for educational purposes only and does not constitute medical advice, diagnosis, or treatment. These peptides are research compounds not approved by the FDA for human use. Always consult qualified healthcare professionals before using any peptide or medication.
References
1. Gwyer D, Wragg NM, Wilson SL. Gastric pentadecapeptide body protection compound BPC 157 and its role in accelerating musculoskeletal soft tissue healing. Cell Tissue Res. 2019;377(2):153-159. https://pubmed.ncbi.nlm.nih.gov/30915550/
2. Chang CH, Tsai WC, Lin MS, Hsu YH, Pang JH. The promoting effect of pentadecapeptide BPC 157 on tendon healing involves tendon outgrowth, cell survival, and cell migration. J Appl Physiol. 2011;110(3):774-780. https://pubmed.ncbi.nlm.nih.gov/21030672/
3. Malinda KM, Sidhu GS, Mani H, et al. Thymosin beta4 accelerates wound healing. J Invest Dermatol. 1999;113(3):364-368. https://pubmed.ncbi.nlm.nih.gov/10469335/
4. Bock-Marquette I, Saxena A, White MD, et al. Thymosin beta-4 activates integrin-linked kinase and promotes cardiac cell migration, survival, and cardiac repair. Nature. 2004;432(7016):466-472. https://pubmed.ncbi.nlm.nih.gov/15565145/
5. Pickart L, Margolina A. Regenerative and Protective Actions of the GHK-Cu Peptide in the Light of the New Gene Data. Int J Mol Sci. 2018;19(7):1987. https://pubmed.ncbi.nlm.nih.gov/30002323/
6. Maquart FX, Pickart L, Laurent M, et al. In vivo stimulation of connective tissue accumulation by the tripeptide-copper complex glycyl-L-histidyl-L-lysine-Cu2+ in rat experimental wounds. J Clin Invest. 1993;92(5):2368-2376. https://pubmed.ncbi.nlm.nih.gov/8227361/

